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. 2018 Nov 19;9:4863. doi: 10.1038/s41467-018-07358-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — FoxO1 bound to HDAC3 and NCoR1 to form a corepressor complex. a Arg1 promoter activity after IL-4 stimulation with or without CA-FoxO1 transfection (n = 4–5). b Mutant A, B, and A plus B Arg1 promoter activities after IL-4 stimulation with or without CA-FoxO1 transfection (n = 5–6). c The binding of DNA nucleotides of the B area of the Arg1 promoter and proteins, and the blocking of the DNA-protein complex formation in a dose-dependent manner by FoxO1, HDAC3 and NCoR1 antibody were determined by EMSA in RAW264.7 cells. d Chip-qPCR using FoxO1, HDAC3 and NCoR1 antibody in the BMDM of the C57BL/6 mice before and after IL-4 stimulation (n = 5–10). e Chip-qPCR using FoxO1, HDAC3 and NCoR1 antibody in the BMDM of the MIrs2KO mice before and after IL-4 stimulation (n = 3–16). f Co-immunoprecipitation (Co-IP) with FoxO1, HDAC3 and NCoR1 antibody without IL-4 stimulation in the Raw 264.7 cells and BMDM. g Expression levels of Arg1 in the BMDM of the C57BL/6 mice after IL-4 stimulation with siHDAC3 or siNCoR1 transfection (n = 3–8). The data are mean ± SEM. followed by one-way ANOVA with a post hoc test. *P < 0.05; **P < 0.01; ***P < 0.001