Fig. 4.

Gene expression profile of repopulating microglia/macrophages. a FACS purities of sorted F4/80^low and F4/80^hi populations. b PCA of microarray expression profiles. n(biological + technical) = 2 GMP, 2 CMP, 3 + 2 Ly6C^hi, 2 RPM, 2 int. mac, 3 + 2 naive microglia, 2 DTR, 3 F4/80^low 4w, 3 F4/80^low 12w, 2 F4/80^hi 4w, 3 F4/80^hi 12w. Naive microglia were sorted from Cx3cr1^CreER/+ mice that received TAM. DTR microglia were sorted from Cx3cr1^CreER/+R26^DTR/+ mice 14 days after DT administration. F4/80^low and F4/80^hi subsets in Cx3cr1^CreER/+R26^DTA/+ mice were sorted 4 and 12 weeks after TAM administration. RPM, int. mac, CMP, GMP, and Ly6C^hi monocytes were sorted from naive mice. Int. mac. intestinal macrophage. Sorting strategies are detailed in the Methods section. Each sample represents pools of 2–5 mice. c Heat map (z-scores) of expression profiles of 15 microglia-specific genes. d Heat map (z-scores) of expression profiles of microglia-specific transcription factors. e Microarray expression counts of Tgfbr1. Lines represent mean values