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. 2018 Nov 19;9(12):1144. doi: 10.1038/s41419-018-1196-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Real-time PCR analyses showing relatively high levels of Rps26 mRNA in GV oocytes and adult testis. The Rps26 mRNA levels of all samples were compared to the Rps26 mRNA level in the lung. b Western blot analyses showing relatively high expressions of Rps26 protein in the adult ovary, testis, and liver. The Rps26 protein levels of all samples were compared to the Rps26 protein level in granulosa cells. Protein levels were quantified with the Image J software, and the average value of each sample was calculated using Microsoft Office Excel. c Representative images of the western blot analysis from b showing the expression of the Rps26 protein in different tissues and organs. d Western blot analysis showing that expression of Rps26 protein in GV oocytes from the ovaries of Rps26^fl/fl/Gdf9-Cre mice was undetectable compared with that in Rps26^fl/fl mice. e mRNA sequencing analysis showing significantly decreased Rps26 mRNA expression in the oocytes of Rps26^fl/fl/Gdf9-Cre mouse ovaries at PD14 and PD28. **p < 0.01 as calculated by two-tailed Student’s t-tests. f, g Immunofluorescence results for the expression of Rps26 in oocytes collected from Rps26^fl/fl mice (f) and Rps26^fl/fl/Gdf9-Cre mice (g). h Cumulative numbers of pups per female of the indicated genotypes. Rps26^fl/fl/Gdf9-Cre females were infertile