Fig. 4. Rps26 is required for RNA synthesis and transcription in growing oocytes.

a The Click-iT assay of the oocytes from PD12–14 mice showing very low 5-EU signal in oocytes from Rps26^fl/fl/Gdf9-Cre mice and high 5-EU signal in oocytes from Rps26^fl/fl mice. b Quantification of oocytes with high and low 5-EU signals in the indicated genotypes from the assay shown in a. c Immunofluorescence of p-S2 showing reduced signal in the nuclei of Rps26^fl/fl/Gdf9-Cre oocytes compared with Rps26^fl/fl oocytes from PD12–14 mice. d, e Western blot assays showing that protein levels of phospho-RNA polymerase II (p-S2) and total RNA polymerase II (RNA pol II) in oocytes (d) and the whole ovaries (e) were decreased in Rps26^fl/fl/Gdf9-Cre mice compared to Rps26^fl/fl mice