Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Nov 13;9:2729. doi: 10.3389/fmicb.2018.02729

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018 Christensen, Hansen, Finster, Christiansen, Nielsen, Otzen and Dueholm.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Methanospirillum hungatei JF-1 sheaths are composed of functional amyloids that require DTT and NaOH for dissolution. (A) Binding of the amyloid-specific antibody WO1 to intact M. hungatei JF-1 (Mhun) filaments (right). The left side is a differential interference contrast image. (B) TEM images of purified sheaths from M. hungatei JF-1 at 8,000X (left) and 25,000X magnification (middle). A zoom in on a spacer plug with its hexagonal arrangement of subunits is shown on the right. Scale bars correspond to 500 nm. (C) Normalized second derivative FTIR spectra of purified sheaths. (D) Gel bands after depolymerization of purified sheaths using DTT and NaOH.