Table 1.
Primer sequences for microbiota 16S V4-5 amplification.
| Name | Forward primer | Reverse primer |
|---|---|---|
| C57 WT1 | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTTAGCTGCCAGCMGCCGCGGTAAT | CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCTGTAGCACCGTCAATTYYTTTRAGTTT |
| C57 WT2 | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTAGCTACCCAGCMGCCGCGGTAAT | CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCTGTAGCACCGTCAATTYYTTTRAGTTT |
| C57 WT3 | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTAGCTACCCAGCMGCCGCGGTAAT | CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCTCACTGTCCGTCAATTYYTTTRAGTTT |
| C57 WT4 | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTAGCTACCCAGCMGCCGCGGTAAT | CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCTATTGGCCCGTCAATTYYTTTRAGTTT |
| C57 WT5 | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTAGCTACCCAGCMGCCGCGGTAAT | CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCTGATCTGCCGTCAATTYYTTTRAGTTT |
| b1.b2 Adr KO1 | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTTAGCTGCCAGCMGCCGCGGTAAT | CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCTTACCATCCGTCAATTYYTTTRAGTTT |
| b1.b2 Adr KO2 | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTAGCTACCCAGCMGCCGCGGTAAT | CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCTCGTGATCCGTCAATTYYTTTRAGTTT |
| b1.b2 Adr KO3 | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTAGCTACCCAGCMGCCGCGGTAAT | CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCTACATCGCCGTCAATTYYTTTRAGTTT |
| b1.b2 Adr KO4 | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTAGCTACCCAGCMGCCGCGGTAAT | CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCTGCCTAACCGTCAATTYYTTTRAGTTT |
| b1.b2 Adr KO5 | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTAGCTACCCAGCMGCCGCGGTAAT | CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCTTCAAGTCCGTCAATTYYTTTRAGTTT |
| b1.b2 Adr KO6 | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTAGCTACCCAGCMGCCGCGGTAAT | CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCTCTGATACCGTCAATTYYTTTRAGTTT |