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. 2018 Nov 19;8:17025. doi: 10.1038/s41598-018-35342-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Representative phase contrast images of inner cell mass (ICM) outgrowths on Ex 96 in control (left panel) and IDL group (right panel). Arrow head marks ICM outgrowths (Scale bar 25 µm). (b) Quantification of ICM area on Ex 96. ICM area was significantly reduced (p < 0.01) in Completely developed ICM Outgrowth (CIO) and Large ICM Outgrowth (LIO) derived from IDL embryos in relation to corresponding control group (c) ICM to trophectoderm (TE) area ratio on Ex 96 was significantly reduced (p < 0.0001) in CIO and LIO derived from IDL embryos compared to control.