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. 2018 Nov 19;8:17033. doi: 10.1038/s41598-018-35324-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Volume rendered images of a coffee berry (berry 2) in the initial stages of colonization by a colonizing female. Lateral sections (a,e,h) according to planes “A”, “B”, and “C” shown in (d). The female and eggs have been digitally removed to show the nest and initial gallery following the entrance hole (a). The components of the berry have been digitally removed to focus on the entrance hole, gallery, colonizing female, and two batches of eggs (b). The female adult and eggs have been digitally removed and the gallery rotated 90° (c) in relation to views in (a) and (b), with the adult and eggs back in place (g). The dotted blue arrows (c) are helpful in visualizing the alternating angles in the gallery following the entrance hole, and at the end, two nests with eggs (a,b,e–i). Detail of the female and eggs inside the galleries and cavities (f); visualization has been rotated 45° in relation to (a); 45° rotation from the petiole, in a distal view (i). Abbreviations: di = disc (style remnant); e1 = 1^st egg batch; e2 = 2^nd egg batch; ed = endocarp (parchment); eh = entrance hole; en = endosperm (seed); ep = epicarp (outer skin); me = mesocarp (mucilage); ne = nest; pe = pedicel; sp = spermoderm (silverskin).