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. 2018 Nov 19;8:17010. doi: 10.1038/s41598-018-34718-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Histological analysis of chondrogenic ASC-pellet cultures under static and hydrostatic pressure stimulated (HP-stim) conditions. Static chondrogenic cultured pellets show an increasing amount of glycosaminoglycan (determined via alcian blue staining) and collagen II (determined via immunohistochemical staining) over time. In contrast, HP-stimulated pellets are negative for these markers but show uniform distribution of collagen I during the full culture time of 42 days. Static pellets also express collagen I, especially at earlier time points, but generally at the outer region of the pellets (indicated by black arrows). Scale bar: 100 µm.