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. 2018 Nov 19;8:17010. doi: 10.1038/s41598-018-34718-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Hydrostatic pressure (HP) stimulation increases gene expression of matrix metalloproteinases proteins. Gene expression of all investigated MMPs (MMP3, MMP9 and MMP13) was significantly upregulated in hydrostatic stimulated pellets on day 21. MMP9 and MMP13 had higher expression in both HP-stimulated groups on day 42 with MMP13 being significantly different to the continuously stimulated group. Data from 3 individual donors, 5 replicates per donor; *p < 0.05, **p < 0.01, ****p < 0.0001.