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. 2018 Nov 19;8:16976. doi: 10.1038/s41598-018-34883-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Release of BDNF-pHluorin from cortical projection neurons close to striatal neurons. (A) Schematic of corticostriatal co-cultures prepared using ibidi 2-well culture inserts and a representative image. (A1) Cortical and striatal neurons were plated in separate compartments and they formed connections after the inserts were removed. A gap area close to the striatal compartment is marked in red. (A2) Representative image showing that a cortical neuron projected to the striatal compartment, in which the imaging area is highlighted by the red box. (B) Response at different times of the cortical projection neuron shown in (A2). (C) Relative fluorescence intensity traces of BDNF-pHluorin at cortical presynaptic terminals close to striatal neurons. (D) Cumulative plot of peak intensity ratio in WT and HD neurons. Inset, bar graph of intensity ratio, showing a significant decrease in HD neurons (n = 61) as compared with that in WT neurons (n = 51); ^***p < 0.001, Mann-Whitney U test. Scale bar: 10 μm.