FIGURE 3.

Effects of glycosylated molecules on the membrane affinity of myristoyl-CM4 to breast cancer cells. Cells were pretreated with the following treatments: sialidase (0.1 U/ml, 30 min), BnGalNac (2 mM, 48 h), tunicamycin (3 μg/ml, 24 h) and L-PPMP (2 μM, 48 h). Then cells were incubated with 3 μM FITC-myristoyl-CM4 for 30 min at 37°C in the dark. (A,C) FITC fluorescence intensity of MX-1 and MCF-7 cells was analyzed by flow cytometry at 488 nm excitation. (B) Reduced fluorescence density was induced by the treatments. (D) Images of MCF-7 cells treated by L-PPMP compared FITC-myristoyl-CM4 (3 μM) with FITC-CM4 (3 μM) were acquired by CLSM observation (excitation, 488 nm; emission, 525 nm). Results are mean ± SEM of 3 different experiments, ^∗∗p < 0.01.