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. 2018 Nov 13;9:1297. doi: 10.3389/fphar.2018.01297

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Copyright © 2018 Li, Liu, Yang, Xu, Lv, Zhang, Liu, Zhang and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Apoptosis detection in breast cancer cells induced by myristoyl-CM4. (A) After treatment by myristoyl-CM4 (4 μM for MCF-7, 6 μM for MDA-MB-231, 3 μM for MX-1) for 16 h, cells were stained by Hoechst 33342/PI and then observed by fluorescence microscopy. (B) Cells were stained by Annexin V/PI and then analyzed by flow cytometry. (C) Lysates were harvested and the apoptotic proteins (cytochrome c, caspase 9, caspase 3, and PARP) were detected by western blotting. (D) The relative amounts of cytochrome c, procaspase 3 and cleaved PARP versus GAPDH were determined by western blotting results and Image J densitometric analysis.