Fig. 5.

TBX2 is a cell dependency gene in neuroblastoma. a Significant reduction in colony area occupancy (%) upon TBX2 knockdown as compared to the non-targeting control (NTC) in CLB-GA cells. b Reduction of proliferation in IMR-32 and CLB-GA cells upon TBX2 knockdown measured with time-lapse microscopy (every 2–3 h) using the IncuCyte device (one out of three biological replicates is shown). c Cell cycle analysis upon TBX2 knockdown in the CLB-GA cell line. Significant induction of G1 cell cycle phase arrest upon TBX2 knockdown (statistical test is based on G1 phase percentage). Data-points were mean-centered (a,c) and error bars represent the s.d. of three biological (a,c) or five technical (b) replicates for every cell line. Mann–Whitney test *p < 0.05, **p < 0.01