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. 2018 Nov 13;9:1580. doi: 10.3389/fpls.2018.01580

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Copyright © 2018 Gagic, Faville, Zhang, Forester, Rolston, Johnson, Ganesh, Koolaard, Easton, Hudson, Johnson, Moon and Voisey.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Visualization of endophyte hyphae and hyphal biomass estimates in tiller crown tissues. Confocal microscopy of longitudinal views through the center of tillers showing hyphae in tiller tissues of a representative (A) high-transmitting parental genotype, 107 and (B) low-transmission genotype, 13 of Population IV. The white dots are labeled septa separating adjacent hyphal compartments (WGA-488, false color white), and scale bar represents 0.2 mm. Scatterplots of the % viable endophyte transmission rates (based on seed harvested at the Grasslands Research Centre in 2013) (C) and % tiller infection rates taken in 2016 (D), relative to endophyte DNA concentration in the shoot apex tissues for a subset of 24 parent plant genotypes from Population IV. Data points for the genotypes shown in panels (A,B) are labeled in red.