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. 2018 Nov 19;9(12):1149. doi: 10.1038/s41419-018-1187-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a, c Apoptosis was detected by flow cytometry in HCT8, HCT116 cells transfected with pcDNA3.1 or pcDNA-H19 (a) and HCT8Fu, SW1116 cells transfected with si-NC or si-H19 (c). The cells were treated with PBS and 5-Fu. b, d Columns are the average of three independent experiments. Data are presented as mean±SD from three independent experiments. **P<0.01, ***P<0.001. e Caspase-3, PARP, and their cleaved forms were detected by western blotting