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. 2018 Nov 19;9(12):1149. doi: 10.1038/s41419-018-1187-4

Fig. 6. H19 sponges miR-194-5p as ceRNA.

Fig. 6

a Schematic illustration of the predicted-binding sites between H19 and miR-194-5p, SIRT1 and miR-194-5p, mutation of potential miR-194-5p binding sequence in H19 and SIRT1. b, c The mRNA expression levels of SIRT1 in HCT8Fu and SW1116 cells transfected with si-H19 (b) and HCT8 and HCT116 cells transfected with pcDNA-H19 (c) were analyzed by qRT-PCR. *P<0.05, **P<0.01, ***P<0.001. d The protein expression levels of SIRT1 in HCT8Fu and SW1116 cells transfected with si-H19 and HCT8 and HCT116 cells transfected with pcDNA-H19 were detected by western blotting. e The relative luciferase activity in HEK-293T cells with and without miR-194-5p overexpression was measured when transfected with pmiR-H19-WT or Mut luciferase plasmids using the dual-luciferase assay. ***P<0.001. f Cellular lysates from HCT8 cells were used for RIP with an anti-Ago2 antibody or IgG antibody. The levels of H19 and miR-194-5p were detected by qRT-PCR. g The relative luciferase activity in HEK-293T cells were cotransfected miR-194-5p and luciferase plasmid containing WT SIRT1 3'UTRs or Mut 3'UTRs with pcDNA-H19 or empty vector was determined using the dual-luciferase assay. **P<0.01, ***P<0.001