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. 2018 Nov 19;9(12):1149. doi: 10.1038/s41419-018-1187-4

Fig. 7. H19 sponges miR-194-5p to modulate SIRT1 expression.

Fig. 7

a CRC tumors were subject to qRT-PCR for detecting the correlation between H19 and SIRT1. b, c Western blotting was used to determine the SIRT1 expression level for si-H19 plus miR-194-5p inhibitor cotransfected into HCT8Fu cells, and pcDNA-H19 plus miR-194-5p mimics cotransfected into HCT8 cells. d, f The cell sensitivity of HCT8 (d) and HCT116 (f) transfected with pcDNA3.1 or pcDNA-H19 to 5-Fu was evaluated using the MTT assay upon exposure to the step-up concentration of 5-Fu with or without overexpression of miR-194-5p for 72h. *P<0.05, **P<0.01, ***P<0.001. e, g Apoptosis was detected by flow cytometry in HCT8 (e) and HCT116 (g) cells transfected with pcDNA3.1 or pcDNA-H19 with or without overexpression of miR-194-5p and treated with 5-Fu. ***P<0.001