Fig. 1.

Adaptation of S. multivorans to pyruvate-fermenting conditions. a Growth curves with pyruvate as sole growth substrate after three and 25 transfers; a culture with pyruvate/fumarate after three transfers is shown for comparison. b Growth during continuous transfer on pyruvate without electron acceptor. Each transfer (10% inoculum) was done after 48 h cultivation. Data were obtained from at least two independent biological replicates and are representatives; graphs of the replicates are shown in Supplementary Figure 2. T number of transfer step, Pyr pyruvate, Fum fumarate, OD₅₇₈ optical density at 578 nm