Figure 3.

KO mice tended to show resistance to fatty liver. (A) The weights of organs at 19 months of age (n = 16–17/group). WT fat weight and KO fat weight were compared with the Welch’s t test. The weights of liver, pancreas, spleen, and kidney between WT and KO were compared with the Student’s t test. *p < 0.05, **p < 0.01 vs. WT group. (B) Hematoxylin and eosin staining of liver (a,g), intra-abdominal WAT (b,h), interscapular BAT (c,i), and islets (d,j) of WT (a–d) and KO (g–j) mice at 19 months of age. Immunostaining for β-cells (insulin staining) (e,k), and α-cells (glucagon staining) (f,l) of WT (a–f) and KO (g,h) mice at 19 months old. Scale bar: 100 µm.