Figure 5.

Maternal high fat diet disrupted intestinal barrier function in 3-week old offspring mice. Barrier formation was detected using the in vivo FITC-dextran assay. The FITC-dextran level in serum is shown (A). Total RNA was extracted from the colonic tissues for real-time PCR analysis. The relative expression of CLDN 1, 3, Occludin, and ZO-1 was shown (B). Protein levels of ZO-1 and CLDN3 in the colonic tissues from 3-week old offspring mice were detected by western blotting and the relative intensity was quantified (C). The membrane localization of ZO-1 was assessed by immunostaining and visualized by fluorescence microscopy (red staining; 400 ×), nuclei were stained with DAPI (blue staining) and the red regions indicated ZO-1 (D). MHFD, maternal high fat diet. MCD, maternal control diet. FITC-dextran, fluorescein isothiocyanate conjugated-dextran. In (A–D), n = 6 in each group. ^*p < 0.05, ^**p < 0.01.