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. 2018 Nov 13;9:1306. doi: 10.3389/fphar.2018.01306

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Copyright © 2018 Lee, Coelho, Class, Sern, Bocz and Szumlinski.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of intra-AcbSh MTEP and adult-onset alcohol consumption upon Egr1 expression within the AcbSh. (A) An anatomical depiction of a coronal section through the striatum, highlighting the approximate size and location of the sampling region used to assay the number of Erg1+ cells within the AcbSh (black square). (B) Overall, adult-onset alcohol-drinking control mice exhibited higher Egr1 expression within AcbSh, relative to water-drinking controls. Both 1 μg and 10 μg/side MTEP significantly reduced Egr1 expression compared to vehicle infusion, irrespective of drinking history. (C) Representative micrographs of Egr1 immunostaining within the AcbSh in each of the six treatment groups. ^∗p < 0.05 treatment effect within same drinking group, ⁺p < 0.05 drinking effect within same intracranial treatment group.