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. 2018 Nov 13;9:1625. doi: 10.3389/fpls.2018.01625

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Copyright © 2018 Akhter, Kretzschmar, Nordal, Delhomme, Street, Nilsson, Emanuelsson and Sundström.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In situ hybridization using short DAL19 isoform specific LNA probes. Micrographs of longitudinal sections of bud meristems, v, vegetative; , female; , male; p, central pith; az, apical zone of the shoot apical meristem; pz, peripheral zone of the meristem. Micrographs (A–D) are hybridized with an LNA-probe that specifically binds to DAL19_δ-containing transcripts. Sections in micrographs (E–H) are hybridized with a DAL19_γ probe, (I,J) with a DAL19_α probe, and (K,L) with a DAL19_β probe. Size bar = 50 μm in (A,B,D–F,H–L), 20 μm in (C,G).

Inline graphic — In situ hybridization using short DAL19 isoform specific LNA probes. Micrographs of longitudinal sections of bud meristems, v, vegetative; , female; , male; p, central pith; az, apical zone of the shoot apical meristem; pz, peripheral zone of the meristem. Micrographs (A–D) are hybridized with an LNA-probe that specifically binds to DAL19_δ-containing transcripts. Sections in micrographs (E–H) are hybridized with a DAL19_γ probe, (I,J) with a DAL19_α probe, and (K,L) with a DAL19_β probe. Size bar = 50 μm in (A,B,D–F,H–L), 20 μm in (C,G).