Figure 1.

Larger functional TRPM8 expression in UM 92.1 than PM cells. Drug additions were made at the indicated time points (arrows). Data are mean ± SEM of 4–8 experiments. (A) CAP (20 μM) induced an irreversible Ca²⁺ influx (n = 4) whereas non-treated control cells showed a constant Ca²⁺ baseline (n = 4). (B) The same effect could be observed in normal porcine melanocytes but with a time lag (n = 7; controls n = 11). (C) Summary of the experiments with CAP (n = 4–7). The asterisks (*) show significant differences between control and CAP (n = 4; 350s; *p < 0.05; n = 7; 590 s; **p < 0.01; paired tested). The hashtag shows a significant difference of the CAP effect at 350 s between UM 92.1 cells and melanocytes (n = 4-7; 590 s; ^#p < 0.05; unpaired tested). (D–F) Same experiments as shown in (A–C), but with icilin (20 μM) (n = 4–8). The icilin effect was markedly reduced in porcine melanocytes. The asterisks (*) show significant differences between control and icilin (n = 4; 350 s; *p < 0.05; paired tested). The hashtags (#) shows a significant difference of the icilin effect at 350 s and 590 s between UM 92.1 cells and melanocytes (n = 4–8; 350 s; ^###p < 0.005; 590 s; ^##p < 0.01; unpaired tested).