Figure 6.

3-T₁AM elicits increases in whole-cell currents through TRPM8 channels in UM 92.1 cells. (A) Time course recording showing the current increases induced by 3-T₁AM and reduction after application of 20 μM AMTB. (B) Original traces of 3-T₁AM -induced current responses to voltage ramps. Current densities are shown before application (labeled as A), during application of 3-T₁AM (labeled as B), and after addition of AMTB (labeled as C). (C) Summary of patch-clamp experiments with 3-T₁AM and AMTB. The asterisks (**) indicate statistically significant increase with 3-T₁AM (n = 9; p < 0.05 at the minimum; paired tested) and decreases in the presence of AMTB (n = 7; p < 0.05; paired tested).). (D) Maximal negative current amplitudes induced by a voltage step from 0 to −60 mV are depicted in percent of control values before application of 1 μM 3-T₁AM. 3-T₁AM-induced inward currents were clearly suppressed in the presence of 20 μM AMTB (n = 4; *p < 0.05). (E) Maximal positive current amplitudes induced by a voltage step from 0 to +130 mV are depicted in percent of control values before application of 1 μM 3-T₁AM. 3-T₁AM-induced outwardly rectifying currents (n = 5; **p < 0.01) were clearly suppressed in the presence of 20 μM AMTB (n = 4; *p < 0.05).