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. 2018 Nov 13;11:388. doi: 10.3389/fnmol.2018.00388

Figure 5.

Figure 5

miR-210 can specifically target on synaptosomal-associated protein of 25KDa (Snap25; n = 3/group, 2 images per sample). (A) The target sites of miR-210-5p in Snap25 mRNA 3’ untranslated region (3’UTR) were analyzed by Targetscan 7.0. (B) The construction profile of psiCHECK2-Snap25 wild-type (WT) and Mut vectors are shown above. Snap25 luciferase reporter (Snap25 3’ UTR WT) and the corresponding mutant construct (Snap25 3’ UTR Mut) were co-transfected into PC12 cells together with miR-210 mimics and inhibitor respectively. Luciferase activity was measured 24 h post transfection. Values are expressed relatively to the internal firefly luciferase activity. (C) Immunofluorescence and its intensity analysis showed miR-210-5p mimics decreased Snap25 protein expression in Primary neuron cells, whereas miR-210-5p inhibitor increased snap 25 protein expression. (D) Western blot results showed a decrease of Snap25 protein expression in primary neuron cells treated with miR-210-5p mimics, whereas they showed an increase of snap 25 expression in cells with miR-210-5p inhibitor. Data are shown as mean ± SD values, and statistical significance between both groups is defined as *P < 0.05; **p < 0.01.