Table 1.
Gene-special primer sequences used for quantitative real-time PCR analysis.
| Gene | Reference sequence | Primer sequences (5′–3′) |
|---|---|---|
| HSP27 | NM_205290.1 | Forward: ACACGAGGAGAAACAGGATGAG |
| Reverse: ACTGGATGGCTGGCTTGG | ||
| HSP40 | NM_001199325.1 | Forward: GGGCATTCAACAGCATAGA |
| Reverse: TTCACATCCCCAAGTTTAGG | ||
| HSP60 | NM_001012916.1 | Forward: AGCCAAAGGGCAGAAATG |
| Reverse: TACAGCAACAACCTGAAGACC | ||
| HSP70 | NM_001006685.1 | Forward: CGGGCAAGTTTGACCTAA |
| Reverse: TTGGCTCCCACCCTATCTCT | ||
| HSP90 | NM_001109785.1 | Forward: TCCTGTCCTGGCTTTAGTTT |
| Reverse: AGGTGGCATCTCCTCGGT | ||
| TNF-α | NM_204267 | Forward: GCCCTTCCTGTAACCAGATG |
| Reverse: ACACGACAGCCAAGTCAACG | ||
| COX-2 | NM_001167718 | Forward: TGTCCTTTCACTGCTTTCCAT |
| Reverse: TTCCATTGCTGTGTTTGAGGT | ||
| PTGEs | NM_001194983.1 | Forward: GTTCCTGTCATTCGCCTTCTAC |
| Reverse: CGCATCCTCTGGGTTAGCA | ||
| NF-κB | NM_205134 | Forward: TCAACGCAGGACCTAAAGACAT |
| Reverse: GCAGATAGCCAAGTTCAGGATG | ||
| IFN-γ | NM_205149.1 | Forward: GCTGACGGTGGACCTATTATTGTAGAG |
| Reverse: TTCTTCACGCCATCAGGAAGGTTG | ||
| iNOS | NM_204961.1 | Forward: CCTGGAGGTCCTGGAAGAGT |
| Reverse: CCTGGGTTTCAGAAGTGGC | ||
| IL-4 | NM_001007079.1 | Forward: GAGAGCCAGCACTGCCACAAG |
| Reverse: GTAGGTCTGCTAGGAACTTCTCCATTG | ||
| GAPDH | NM_204305.1 | Forward: GCACGCCATCACTATCTTCC |
| Reverse: CATCCACCGTCTTCTGTGTG |