Fig. 1.

Amplification by real-time PCR signals on C. parvum (Cp), G. lamblia (Gl), C. cayetanensis (Cc), and bacteriophage T4 (BpT4) chosen target genes using a primer-probe cocktail. The mixture of 4 primer pairs and 4 kinds of probes was prepared in advance and reacted with the DNA mixture of Cp, Gl, Cc, and Bp using the quadroplex real-time PCR method. Fluorescent dyes used in present study were as follows. Cp (blue line): FAM^™; Gl (green line): HEX^™; Cc (purple line): Cy5^™; Bp (red line): CAL Fluor Red^® 610.