Fig. 2.
Standard curve of protozoa genomic DNA amplification by real-time PCR using a primer-probe cocktail. The 10-fold serial diluted (103, 104, 105, and 106) genomic DNA was extracted from oocyst of Cp (A) and cyst of Gl (B). The 10-fold serial diluted concentration (106, 105, 104, and 103) genomic DNAs of Cc (C) and Bp (D) were prepared from purchased Cc DNA and T4 phage, respectively. Cp (blue line): FAM™; Gl (green line): HEX™; Cc (purple line): Cy5™; Bp (T4 phage, red line): CAL Fluor Red® 610.