Fig. 3.

Limit of detection (LOD) for each parasite in parasite-spiked human stool samples using the multiplex real-time PCR assay method. LOD of each parasite was investigated with a primer-probe cocktail and determined by Ct values. BpT4 (10⁷ CFU) used as an internal control were also spiked with each parasite in the stool sample. (A) Cp (blue line) and Bp (red line). (B) Gl (green line) and Bp (red line). (C) Cc (purple line) and Bp (red line).