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. 2018 Oct 30;115(46):11838–11843. doi: 10.1073/pnas.1814434115

Fig. 2.

Fig. 2.

RLP44 and BRI1 are required for the control of xylem cell fate. (A) Overview of xylem differentiation in the Arabidopsis root and schematic representation of the stele. Gray square in root schematic indicates point of xylem observation. (B) Basic fuchsin staining of 6-d-old Arabidopsis root. DIC image shows secondary cell-wall thickenings of protoxylem and metaxylem (Left), and basic fuchsin labels lignified secondary cell walls (Middle). Confocal stacks allow xylem number quantification of the indicated genotypes in orthogonal view (Right). Note ectopic metaxylem in procambial position (arrow). (Left) A median plane image. (Middle) A maximum projection. (Scale bar: 50 µM.) (C and D) Frequency of roots with the indicated number of metaxylem cells in rlp44 and BR-related mutants. Right in D shows orthogonal view and maximum projection of bri-triple root. Note ectopic metaxylem (arrows) and disrupted protoxylem (arrowhead). Asterisks indicate statistically significant difference from Col-0 based on Dunn’s post hoc test with Benjamini–Hochberg correction after Kruskal–Wallis modified U test (*P < 0.05; ***P < 0.001). (E) Transgenic expression of BRI1 under control of its own regulatory 5′ sequence rescues the ectopic xylem phenotype of bri1-null.