Fig. 1.
GRSD molecules fold and unfold reversibly in single-molecule experiments. (A) Scheme of optical-trap assay. DEX bound GR-LBD F602S [Protein Data Bank (PDB) ID code 1M2Z] linked to dsDNA linkers which are tethered to 1-µm silica beads. (B) Stretch (blue) and relax (orange) cycle in the presence of 200 µM DEX using a constant pulling velocity of 500 nm/s. Dashed colored lines show WLC fits for different contour length (details in SI Appendix). (C) Contour length vs. time trace of the transition between open (blue) and closed (purple) state while keeping the traps at a constant distance applying a force bias of around 10 pN (passive-mode). (D) Force dependence of closing (blue) and opening (purple) rates. Lines are extrapolations using a model described in SI Appendix.