Fig. 2.

Release of ATP by E. coli cells encoding ADP/ATP translocase. (A) Cellular [γ-³⁵S]ATP uptake/release by E. coli cells expressing the UWE25 ADP/ATP translocase (pAM94 plasmid) in the presence of 1 mM arabinose. Cellular [γ-³⁵S]ATP was released when E. coli cells expressing the ADP/ATP translocase were challenged with extracellular ADP (10 mM), but not with phosphate (Pi) or AMP (each at 10 mM). (B) Release of ATP into the growth medium by E. coli cells expressing the UWE25 ADP/ATP translocase (pAM94 plasmid) in presence of 20 µM ADP and 1 mM arabinose. The ATP concentration in the medium was determined by luciferase assay. Data bars show a mean of three technical replicates; error bars represent SE of the mean.