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. 2018 Oct 14;5(11):1394–1407. doi: 10.1002/acn3.669

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© 2018 The Authors. Annals of Clinical and Translational Neurology published by Wiley Periodicals, Inc on behalf of American Neurological Association.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Skin innervation: intraepidermal nerve fiber density (IENFD). Immunofluorescence staining with anti‐PGP9.5 (green) and DAPI (blue) of cervical skin in an healthy subject (A) and PD (C); in A the dotted white line shows the border between epidermis and dermis: IENFD is calculated as the number of nerve fibers (arrows) crossing the border per mm. Dermal nerve fibers around sweat glands are shown in an healthy subject (B) and PD (D). The pictures shows skin denervation in PD more evident at level of autonomic nerve fibers of sweat gland. Scale bar 50 μm.