Table 1. pAS Plasmids for EF1α Controlled Expression of PylS and Reporter Genes.
| plasmid number | 4 × tRNA cassette | EF1α promoter |
|---|---|---|
| 1 | – | FLAG_Mma PylRS |
| 2 | 7SK-Mma PylT | FLAG_Mma PylRS |
| 3 | 7SK-Mma PylT | FLAG_Mma PylRS/AF |
| 4 | 7SK-Mma PylT | FLAG_Mma PylRS CTD |
| 5 | 7SK-Mx1201 PylT C41CA | FLAG_Mma PylRS |
| 6 | 7SK-Mma PylTUUA | FLAG_AcKRS |
| 7 | – | FLAG_Mx1201 PylRS |
| 8 | U6-Mx1201 PylT | FLAG_Mx1201 PylRS |
| 9 | U6-Mx1201 PylT | FLAG_Mx1201 PylRS Y126A |
| 10 | 7SK-Mx1201 PylT | FLAG_Mx1201 PylRS |
| 11 | 7SK-Mx1201 PylT | FLAG_Mx1201 PylRS Y126A |
| 12 | 7SK-Mx1201 PylT C41CA | FLAG_Mx1201 PylRS |
| 13 | 7SK-Mx1201 PylT C41CA | FLAG_Mx1201 PylRS Y126A |
| 14 | – | sfGFP 150TAG |
| 15 | 7SK-Mma PylT | sfGFP 150TAG |
| 16 | 7SK-Mma PylTUUA | sfGFP 102TAG 150TAA |
| 17 | U6-Mx1201 PylT | sfGFP 150TAG |
| 18 | 7SK-Mx1201 PylT | sfGFP 150TAG |
| 19 | 7SK-Mx1201 PylT | HA histone H3.2 K56TAG |
| 20 | 7SK-Mx1201 PylT C41CA | sfGFP 150TAG |
| 21 | 7SK-Mx1201 PylT C41CA | sfGFP 102TAG 150TAA |