Assessment
of the hematological toxicity of imidazotetrazines in vivo. Mice were administered a single IV dose of 125
mg/kg imidazotetrazine. After 7 days, whole blood was collected, and
a complete blood count was obtained for each individual mouse. (a)
Total WBC count. Control vs Ox-TMZ: P = 0.7, Control
vs K-TMZ: P = 0.9. (b) Lymphocyte concentrations.
Control vs Ox-TMZ: P = 0.5, Control vs K-TMZ: P = 0.9. Error is SEM, number of mice per cohort = 4. Statistical
significance was determined by using a two-sample Student’s t test (two-tailed test, assuming equal variance). The concentrations
of other relevant blood constituents can be found in Supporting Information Figure S3.