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. 2018 Nov 13;8:397. doi: 10.3389/fcimb.2018.00397

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Copyright © 2018 Boussoffara, Chelif, Ben Ahmed, Mokni, Ben Salah, Dellagi and Louzir.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Timeline showing progress of the prospective study. Four hundred and fifty three participants living in endemic foci of zoonotic cutaneous leishmaniasis (ZCL) in Central and Southwestern Tunisia (Gafsa and Sidi Bouzid) were followed up throughout one season of L. major transmission. Several parameters such as leishmanin skin test (LST) or the presence of typical ZCL scar were monitored at the beginning of the study. Peripheral blood samples were obtained from each donor and will be used to analyze host cytotoxic immune response. Repeated clinical examinations were done to diagnose the new cases of ZCL emerging after one transmission season (September 2001–April 2002).