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. 2018 Nov 13;8:397. doi: 10.3389/fcimb.2018.00397

Figure 2.

Figure 2

Granzyme B levels induced in response to stimulation with Leishmania major. (A) Grz B is measured in culture supernatants of PBMCs incubated for 5 days with live promastigotes L. major at a ratio of 3 promastigotes per cell. We used PBMCs from patients with active ZCL, healed ZCL subjects or from healthy individuals living in endemic focus for transmission of L. major or outside these areas. Horizontal bars represent median values. (B) Histograms represent the number of subjects categorized in Grz B+ and Grz B−, basing on the cut-off fixed at 2,000 pg/mL. (C) Association between Grz B and IFN-γ levels measured in the same supernatants (D,E) Association between Grz B levels and results of leishmanin skin test (LST), expressed as diameter of induration and the lymphoproliferative response to soluble Leishmania antigens (SLA), expressed as Δcpm. Horizontal dashed lines indicate cut-off for Grz B level, LST and proliferation fixed at 2,000 pg/ml, 5 mm and 5,000 cpm, respectively.