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. 2018 Nov 13;9:1610. doi: 10.3389/fphys.2018.01610

Table 1.

Technical details for recombinant OBP protein expressions.

Protein Vector Expected MW (kDa) Cloning strategy
OBP25 pET30a ~15.4 NdeI-OBP25-His tag-Stop codon-HindIII
OBP30 pET30a ~13.8 NdeI-OBP30-His tag-Stop codon-HindIII
OPB16 pET30a ~18.5 NdeI-OBP6-His tag-Stop codon-HindIII
OBP8 pET30a ~14.4 NdeI-OBP8-His tag-Stop codon-HindIII
OBP4 pET30a ~12.8 NdeI-OBP4-His tag-Stop codon-HindIII