Table 2.

Outputs from Ion PGM and MiSeq platforms during the field study.

Food category	Food sub-category	Observed occurrence in the Ion PGM run			Observed occurrence in the MiSeq run			Observed occurrence*
		in one replicate	in two replicates	Total	in one replicate	in two replicates	Total
Prey items	Small/medium-sized rodents	88	133	221	119	111	230	271
	Insectivores	1	3	4	7	2	9	10
	Wild birds	3	3	6	23	13	36	37
	Reptiles	0	0	0	1	0	1	1
	Total prey items	92	139	231	150	126	276	319
Human-linked food	Game species	4	1	5	8	6	14	15
	Anthropogenic food	117	53	170	289	151	440	187 (440)
	Total human-linked food	121	54	175	297	157	454	202 (455)
Total		213	193	406	447	283	730	521 (774)

Observed occurrences of food items in 326 field-collected faecal samples from free-ranging cats. The observed occurrences are shown according to the HTS platform used and the PCR replicates. The final observed occurrence corresponds to the pooled results from the Ion PGM and MiSeq runs. See Supplementary Table S2 for more details about food sub-categories.

*One or more than one anthropogenic food taxa can be identified in one faecal sample, i.e. multiple occurrences, without being able to associate taxa to independent ingestion events (pet food is composed by a mix of meat and/or fish belonging to various taxa), conversely to prey taxa and game species occurrences. Multiple occurrences are indicated in brackets.