Figure 5. Lysis functional assay in hCD59 mutants.

WT and mutant constructs Cys64Tyr, Asp24Val, Asp24Valfs*, and Ala16Alafs* were transfected to CHO cells. Forty-eight hours after transfection, cells were marked by calcein AM, and calcein fluorescence of supernatants was read using the Cytation 3 Cell Imaging Multi-Mode Reader with the excitation filter set at 485 nm and emission filter at 530 nm. Percent lysis for each well was calculated as calcein release/total calcein loading (*p < 0.05, **p < 0.002, ANOVA and Student t test). WT = wild type.