Agarose gels electerophoresis of Shiga toxin-producing Escherichia coli isolates. (a) Polymerase chain reaction amplification of the stx1 (551 bp) and stx2 (118 bp) genes. Lanes 1–3, stx1; lanes 1–3 and 5, stx2 and (b) polymerase chain reaction amplification of the eae gene (840 bp) (lanes 1–4, 6 and 7). Lane M, 100 bp molecular size markers; Lanes C- and C+, negative and positive control.