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. 2018 Nov 20;8:17071. doi: 10.1038/s41598-018-34949-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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pH 5.5 is the optimal pH for transient GUS expression of Col-0 and efr-1 seedlings. Arabidopsis Col-0 and efr-1 seedlings were infected with A. tumefaciens C58C1(pTiB6S3ΔT)^H (OD₆₀₀ = 0.02) harbouring pBISN1 in AGROBEST medium with buffered pH (5.0, 5.5 and 6.0) and were compared by quantitative GUS activity assay (a) and GUS staining (b) at 3 dpi. Data are mean ± SD GUS activity from three biological replicates (~10 seedlings per well, three wells). Similar results were obtained from three independent experiments. Significantly different values are denoted (*P < 0.05 by Student t test).