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. 2018 Nov 20;4:105. doi: 10.1038/s41420-018-0124-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Chemical structure of apigenin. b, c MDA-MB-231 and MDA-MB-436 cells were treated with apigenin (0–64 μM) for 24, 48, and 72 h, and cell viability was measured via the SRB assay. IC50 values were calculated by Prism 6 software. d Representative images of colony formation assay of the two cell lines. MDA-MB-231 and MDA-MB-436 cells were plated in 24-well plates and treated with indicated concentration of apigenin for 72 h, and then replaced for fresh medium. After 10 days, the plates were stained with 0.2% crystal violet and counted. e Summary of the number of colonies in MDA-MB-231 and MDA-MB-436 cells. The results shown are representative of at least three independent experiments. Data are shown as the mean ± S.D. (*P < 0.05 and **P < 0.01 compared with the control group)