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. 2018 Nov 20;8:17111. doi: 10.1038/s41598-018-35383-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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A recombinant protein comprising amino acids 1 to 79 of human PRL inhibits the migration of endothelial cells and the endothelial cell expression of vasoinhibin target genes. (a) Bovine pulmonary artery endothelial cell (CPAE) monolayers were scratched and treated for 24 h with medium alone (Ctl) or containing transduced HEK293T/17 conditioned media without (Neg) or with the Vi79 or the Vi123 residue vasoinhibins. Representative images of wound sealing at 0 and 24 h. Scale bar, 100 μm. (b) The level of migration into the wound scratch was quantified as the percentage of wound sealing at 24 h relative to respective group value at onset (0 h). Values are means ± SEM of 3 independent experiments (*p < 0.001). (c) Expression of vasoinhibin target genes interleukin-1α (IL1A) and intercellular adhesion molecule 1 (ICAM1) measured by quantitative real-time PCR (qRT-PCR) normalized against cyclophilin A (PPIA). CPAE were treated for 4 h with medium alone (Ctl) or containing conditioned media without (Neg) or with Vi79 or Vi123.Values are means ± SEM of 3 independent experiments (*p < 0.001).