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. 2018 Nov 20;8:17069. doi: 10.1038/s41598-018-35223-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Functional characterisation of ∆ZtPpt and related genes for auxotrophic and stress responses. (A) From top to bottom, WT (ΔZtKu70), ∆ZtPpt-1 and ΔZtPpt-2 grown on YPD agar, YPD agar with added BPS to remove available iron, YPD agar supplemented with the BPS and DFF to remove and then restore available iron, YPD agar grown in darkness, YPD agar after a further six days of exposure to UV light, YPD agar with the ROS species hydrogren peroxide added, CD minimal agar without lysine, CD minimal agar with lysine. (B) From top to bottom, the two strains ΔZtNrps1-1 and ΔZtNrps1-2 grown on YPD agar, YPD agar with added BPS to remove available iron and YPD agar with added BPS and DFF to remove then restore available iron. (C) From top to bottom, the ∆ZtAar strain grown on CD minimal agar with lysine and CD minimal agar without lysine. (D) From top to bottom, the two melanin synthetase strains ∆ZtPks1-1 and ∆ZtPks1-2 after six days of growth on YPD in darkness and a further six days of growth under UV light. All YPD plates were grown for six days in darkness apart from those that underwent an additional six days of exposure to UV light.