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. 2018 Nov 20;8:17127. doi: 10.1038/s41598-018-35431-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Ultrashort peptides are non-hemolytic, even at their solubility limit. When mixed with rabbit red blood cells, the amount of haemoglobin released is comparable to the negative control, PBS. The detergent Triton X100 (1%) is used as the positive (lytic) control. The error bars represent the standard deviation of eight replicates. (b) The peptides are non-cytotoxic. Primary cells (hASCs) cultured in media containing solubilised peptides demonstrated comparable metabolic activity as untreated controls. The error bars represent the standard deviation of 24 replicates.