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. 2018 Nov 20;8:17066. doi: 10.1038/s41598-018-35151-7

Figure 2.

Figure 2

Macrophages from CF patients on Ivacaftor have altered polarization. (A) Baseline M1 and (B) M2 polarization of freshly isolated non-CF and CF monocytes. CF monocytes were grouped according to the presence or absence of clinical CFTR modulator treatment. “*” denotes a p value < 0.05 and “**” denotes a p value < 0.01, one-way ANOVA with post-hoc Tukey. (C) M1 and D) M2 polarization of non-CF and CF MDMs after 48 h exposure to polarizing stimulus. Data were normalized to non-CF MDMs. “*” denotes a p value < 0.05, one-way ANOVA with post-hoc Tukey. The flow cytometry gating strategy for Fig. 2 is found online (Supplementary Fig. S1), M1: CD68/CD80; M2: CD163/CD206.