Table 2.
Orthogonal-array design for phytohormone treatments and their effect on adventitious shoot induction and proliferation.
| Treatment# | Orthogonal arraya | Phytohormone (mg L−1) | Inductionb (%) | Multiplication coefficientb | Adventitious shoot appearance | ||||
|---|---|---|---|---|---|---|---|---|---|
| A | B | C | 6-BAP | NAA | KT | ||||
| 1 | 1 | 1 | 1 | 0.2 | 0.2 | 0.5 | 68.3 ± 2.9 de | 2.8 ± 0.3 e | healthy and strong, bottle green |
| 2 | 1 | 2 | 2 | 0.2 | 0.4 | 1.0 | 81.7 ± 2.9 bc | 3.8 ± 0.4 c | healthy and strong, green |
| 3 | 1 | 3 | 3 | 0.2 | 0.6 | 2.0 | 53.3 ± 7.6 f | 3.5 ± 0.2 cd | thin and delicate, yellow green |
| 4 | 2 | 1 | 2 | 0.5 | 0.2 | 1.0 | 93.3 ± 2.9 a | 5.3 ± 0.3 a | healthy and strong, bottle green |
| 5 | 2 | 2 | 3 | 0.5 | 0.4 | 2.0 | 80.0 ± 5.0 bc | 4.7 ± 0.3 b | thin and delicate, yellow green |
| 6 | 2 | 3 | 1 | 0.5 | 0.6 | 0.5 | 71.7 ± 2.9 cd | 3.0 ± 0.2 e | healthy and strong, green |
| 7 | 3 | 1 | 3 | 1.0 | 0.2 | 2.0 | 61.7 ± 7.6 ef | 2.9 ± 0.3 e | thin and delicate, yellow green |
| 8 | 3 | 2 | 1 | 1.0 | 0.4 | 0.5 | 73.3 ± 7.6 bcd | 3.2 ± 0.3 de | healthy and strong, green |
| 9 | 3 | 3 | 2 | 1.0 | 0.6 | 1.0 | 83.3 ± 5.8 b | 4.4 ± 0.4 b | healthy and strong, yellow green |
aNumbers represent the levels in orthogonal array of 3 × 3 design; bvalues are average and SD of three independent experiments and different letters indicate significant differences (P < 0.05), as determined by one-way analysis of variance (ANOVA) with Duncan's post-test.