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. 2018 Nov 20;8:17085. doi: 10.1038/s41598-018-35286-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RT-qPCR validation of the RNA-Seq transcriptomic data. Chart shows correlation of fold changes for six genes’ expression from B. longum BBMN68 cells after 30 min (squares) or 60 min (diamonds) exposure to 3% (v/v) oxygen, as derived from the transcriptomic analysis and RT-qPCR. The best fit is shown along with the calculated equation and r² value.